Introduction
- Respiration = Biochemical process of releasing energy (ATP) from organic compounds (mainly glucose).
- Occurs in all living cells.
- Opposite to photosynthesis.
- Energy is released in a stepwise, controlled manner (not explosive).
- ATP is the universal energy currency.
Equation (Aerobic respiration):
Types of Respiration
1. Aerobic Respiration
- Requires oxygen.
- Complete oxidation of glucose → CO₂ + H₂O.
- Occurs in mitochondria.
- Produces maximum ATP (36–38 ATP per glucose).
Major steps:
- Glycolysis (cytoplasm).
- Pyruvate oxidation (link reaction).
- Krebs cycle (mitochondrial matrix).
- Electron Transport System (inner mitochondrial membrane).
2. Anaerobic Respiration (Fermentation)
- No oxygen required.
- Incomplete breakdown of glucose.
- Occurs in cytoplasm.
- Produces 2 ATP only per glucose.
Pathways:
Alcoholic fermentation (yeast, some bacteria):Glycolysis (Embden–Meyerhof Pathway)
- First step of both aerobic and anaerobic respiration.
- Occurs in cytoplasm.
- Glucose (6C) → 2 Pyruvate (3C).
- Net products: 2 ATP + 2 NADH.
Pyruvate Fate
- Aerobic: → Acetyl CoA (enters Krebs cycle).
- Anaerobic: → Lactic acid or ethanol.
Krebs Cycle (Citric Acid Cycle / TCA Cycle)
- Discovered by Hans Krebs.
- Occurs in mitochondrial matrix.
- Starts with Acetyl CoA (2C) + Oxaloacetate (4C) → Citrate (6C).
Steps (simplified):
- Acetyl CoA + OAA → Citrate (6C).
- Citrate → Isocitrate.
- Isocitrate → α-ketoglutarate (5C) + CO₂ + NADH.
- α-ketoglutarate → Succinyl CoA (4C) + CO₂ + NADH.
- Succinyl CoA → Succinate + GTP (→ ATP).
- Succinate → Fumarate + FADH₂.
- Fumarate → Malate.
- Malate → Oxaloacetate + NADH.
Net gain (per Acetyl CoA):
- 3 NADH
- 1 FADH₂
- 1 GTP (≈1 ATP)
- 2 CO₂
Since 1 glucose → 2 Acetyl CoA, total yield = 6 NADH, 2 FADH₂, 2 ATP, 4 CO₂.
Electron Transport System (ETS) and Oxidative Phosphorylation
- Occurs in inner mitochondrial membrane (cristae).
- Involves a series of electron carriers:
Key points:
- NADH donates electrons at Complex I (produces 3 ATP).
- FADH₂ donates at Complex II (produces 2 ATP).
- O₂ is the final electron acceptor → forms H₂O.
- Chemiosmosis: Protons pumped into intermembrane space; flow back via ATP synthase, generating ATP.
Energy Yield Summary (Aerobic Respiration of 1 Glucose)
| Step | ATP/NADH/FADH₂ | ATP Equivalent |
|---|---|---|
| Glycolysis | 2 ATP + 2 NADH | 2 + 6 = 8 ATP |
| Pyruvate oxidation (Link) | 2 NADH | 6 ATP |
| Krebs Cycle | 2 ATP + 6 NADH + 2 FADH₂ | 2 + 18 + 4 = 24 ATP |
| Total | – | 38 ATP (max) |
(Some books mention 36 ATP due to transport losses.)
Anaerobic vs Aerobic Respiration – Quick Comparison
| Feature | Aerobic | Anaerobic |
|---|---|---|
| O₂ requirement | Yes | No |
| Site | Cytoplasm + Mitochondria | Cytoplasm only |
| End products | CO₂ + H₂O | Ethanol + CO₂ / Lactic acid |
| ATP yield | 36–38 | 2 |
| Efficiency | High | Low |
Respiratory Quotient (RQ)
- Carbohydrates = 1
- Fats < 1 (≈0.7)
- Proteins ≈ 0.8
- Anaerobic respiration = ∞ (since O₂ = 0)
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